I have a PhD in molecular biology with focus on stem cells...

I have a PhD in molecular biology with focus on stem cells. Ask me anything about the more than ten years I wasted in science.

how can imicroewave a pancake properly

Wasted? Explain.

Your ideas on how to safely store Antimatter atoms for longer periods of time

Use a metal plate. Pancakes don't need to be cooked, they need to be zapped.

Poor, overqualified and unemployed.

When did you learn that for every veil you remove there will always be another one behind it.

Tupperware.

During my exchange to Saudi-Arabia.

Why and What are lobotomys for?

You're breaking my heart OP. What's to be done about it?

Got any research papers you'd like to share? I'm currently a senior undergraduate biology mayor so I'm interested

Get a job you loon

why is there still no proper cure for hairloss?

whem will humans live forever?

Just learn to write software, its not exciting but the pay is good and companies are so desperate for software developers they'll snap you up in a heart beat.

Maybe write software that does biology shit for demo purposes and show them during the interview.

Inventing a time machine and studying something useful.

I don't want to be identified.

That's what I've been trying the last six months.

Tell me the most amazing fact that you can think of.

Why did you decided to go with STEM studies anyways?

pic unrelated, meme to cheer you up

What do you think of those pajeets that claim to be able to cure diseases such as duchenne muscular dystrophy by injecting stem cells into muscles?

On a related note, do you think such treatment would be more effective on someone who also had a full analogous bone marrow transplant?

My bad.
Then could you vaguely describe one of them for us?

>why is there still no proper cure for hairloss?

Because it is linked to systemic hormonal changes which cannot be modulated without significant side effects.

I spend at least two years of PhD analysing gene expression data with shitty, self-made Python scripts. That didn't impress any employer yet though.

whats the best broth for lymphocytes?
and does is rly matter wehn the surrounding air has 4 percent co2 and not 5

I spent the last 4-5 years doing a PhD in Bioinformatics / Epigenetics. The summary of my thesis would have been "The software and dataformats are so bad much of the data produced by the field is probably wrong."

Got kicked out of the institute after 4.5 years for taking the .5 too long when writing up my thesis. Had a mental breakdown, life is a lie, etc. Now starting an international union for scientists off the money i earned from Bitcoin.

Gonna bring it all down i hope :-l

These unregulated stem cell clinics that claim to cure all kinds of stuff by injecting either autologous stem cells harvested from blood (e.g. MSCs), tissue stem cells or undifferentiated or in vitro differentiated ipSCs are mostly hacks. Sounds nice and scientific but is not so easy in reality.

What are they actually doing in israel? Is desert people conflict contrived as a means to collect endless stem cells in order to keep their diseased gene pool going?

>"The software and dataformats are so bad much of the data produced by the field is probably wrong."

Sounds about right. Oftentimes, even more basic shit is wrong such as faulty statistics. absence of any statistical power, replication issues etc. I've seen so many nature papers where some stupid hypothesis was validated with comics data consisting of one ctrl and one treatment replicate.

Have you ever heard of something that increased the rate of hybridization in muscles after a bone marrow transplant? Or anything that increased the rate of new cell growth normally in muscle tissue, because I would imagine that would cause such a thing.

Yup. Now what the fuck do we do -_-

Which programs give most profit? Where to learn?

Would be nice to travel the world and work on a lap top.

Jesus dude. Thats ruff. It will get better, i´m with you in my thoughts.

Spit or swallow?

Programs? I Don't know, probably something that gets people to be narcissists in a whole new way in front of strangers and friends.

Also, forget travelling around the world while working on a laptop, if you want stability, get a 9 to 5 like the rest of us.

There are certainly a number of pathways which would induce TFs integral to muscle cell differentiation. When it comes to diseases like DMD, the problem is that such an increase in SC turnover to increase the amount of differentiated muscle cells might eventually deplete the stem cell pool altogether.

Dude,

Just finished my masters degree in geology. I already decided to not push it any further. Thinking about becoming a fitness coach, also teaching Martial Arts and Yoga.

I´m glad i kept my hobbies at a high level while studying. I see others who don´t get a job after graduating and having no other skills.

I know it´s gonna be hard and propebly not well payed in the beginning, but i´m gonna try. I don´t want to work in a fucking office. Would blow my brains out in that numbing hell.

Sorry but what is TFs / SC ?

And if the "stem cell pool" were depleted by such a thing, would repeated transfusions by the same donor help?

So I should stop going this route? Also by 10 years do you include schooling or in the field? If its including schooling you are fine. 5 years post doc work is average I believe, it sucks but its the life PhDs live.

Are you an ex-biologist? What's your field? I could make that happen... but the job involves post-peer reviewing of the literature, so you'd have to read the lit. of your subject area (whatever that is) and be part of a team that debunks it if it's wrong.

Don´t want stability good Sir. At least not now.

tell us something interesting

Calm down Randy

Yah absolutely - it's the live of CURRENT PhDs. It wasn't always like this, and it doesn't have to be...

Ever find out why they killed all the top microbiologists a few years ago?

sounds like a good TV show

Huh?

Yeah but do they not pay decent at first? (30-40k). 5 years after isnt too surprising, MDs do the same with residency and even more if they specialize. Also is industry not easier to get into then academia?

Funnily enough, the model is to advertise through YouTube....

Did you ever want to hold a terry fold?

Tell me more :)

Geologist with main emphasis on organic geochemistry. Wrote my master thesis on lipids in eustigmatophytes (heterokont algae).

Also did a bunch of courses in biology on the side including genetics and microbiology.

Hi OP, I graduated last year with bachelors in biochemistry and molecular biology. Every relevant job declined me for no apparent reason. I felt the whole thing was a waste. I picked up a job that was literally out of highschool tier shit. After 6 months I was able to land a chemist position at that company and ended up making 75k/year. I spend every day now walking past the normies that don't have a degree, I make 2.5x their salaries and they will never make anything near me because of the degree I hold. There's hope, but you need to start somewhere get some kind of employment don't be picky at first.

That's pretty interesting :) OK i'll ask two more questions:

Other than food, a place to sleep, a laptop and an internet connection, are there any bits of specialised equipment you would need in order to do research?

Do you get sea sick and would you enjoy sailing from Australia to Asia for a year? Everyone else on board is a PhD 31 or younger.

What kind of work are you seeking? What inspired you to enter the field?

I insist that since radio waves travel faster than sound waves that me using my walkie talkie to talk to my coworker that is 4 feet away is faster than talking to him directly. Prove me wrong.

Biology- science for people with bad math skills.

The message will be sent faster, but the process of making the transmission will be slower.

Oh you can fuck right off with that. Im a BioChem major and believe me there is plenty of math my friend.

What do mean equipmentwise?

I don´t get sea sick, but don´t know if i would enjoy it for a whole year. Would have to see if i´m up to the task and how we function in a group.

x1 for this.

TF: transcription factor; SC: stem cell

There is a huge problem with the transfusion approach for most tissues. It works great for regeneration of hematopoeitic stem cells (HSCs), but this is due to the fact that the blood and the immune system are (mostly) fluid tissues. Therefore, the transplanted stem cells can easily find their niche (which is furthermore empty). This is not so easy with other tissues. If you inject a muscle stem cell / satellite cell into the blood stream, it would have far bigger problems finding its natural place in the muscle stem cell niche. Homing stem cells to solid tissue over the blood stream is ineffective at best. Injecting stem cells directly into the muscle might help but you would have to inject at a lot of different sites (were probably talking about millions of injections to get all he human skeletal muscle)

Do you believe that the first CRIAPR edited human has been born? If so where? If not when do you think s/he will be?

Most science is bullshit due to the major structural problems in academic culture.

#Truth.

I include active research work which started during my Masters, and also covered my PhD and a poostdoc.

It's the goddamn postmodernists, isn't it?

If only there were a way to grow a mesh over the muscles. Is it correct to say that the TF is what's responsible for hybridization post-bone marrow transplant? Generally speaking, what is done to boost it?

I know you aren't happy with your job prospects in this line of research but I do envy you. As a family member of someone with such a disease, I only wish I had the knowledge & credentials to make a difference.

Why don't we just give PHD to Downs ppl as a systematic track for keeping then busy and looking cute?

>What kind of work are you seeking? What inspired you to enter the field?

Anything really which goes beyond serving fries, waiting or sitting at the cash register. I'm fine with lab tech work, data wrangling and curation etc. Don't even think about positions like R&D scientist, project leader, data analyst anymore.

muscle hybridization that is

Please keep the thread alive. Gonna buy some groceries.

aw man, okay. I have to go too, but thanks for your info regarding SC thearapies.

No. This has nothing to do with the academic left or what you would call it. It simply has to do with the fact that the person who can produce flashy data based on flawed methodology and over-interpret this data to such an extent as to make some outlandish claims for the P&R department to tout will be more successful in science than person who is careful and conservative* in his conclusions (*not in the political sense you faggot).

This is kind of what is happening.

Have you ever tried that website, Nature jobs or whatever it's called? Or working in the industry instead of academia

I´m not OP Bro.

Yes and yes. After my last postdoc was terminated due to funding issues, I stopped considering working in academia altogether.

To all Biology undergrads:
Switch majors, you won't land a well paid job after you graduate. I graduated in 2012 with a Biology degree and the only job I could get was as a janitor in a hospital.
I'm currently in my last year of nursing school and there's already many available jobs. Please, PLEASE do not for the Biology/Chemistry meme, there might be cases where people get well paid jobs after they graduate, but those are rare cases. And for the love of god, do not pursue a masters/PhD in Biology.

How do you feel about Rupert Sheldrake's morphic field model, and his contention that genetics alone doesn't contain enough information to create a functional living creature?

Hey there Sup Forumsros I'm currently a Psych Major and plan on going in Psychiatry..Not sure if this is a good field or not but just wanted to know if anyone had any information on it.

> Got kicked out of the institute after 4.5 years for taking the .5 too long when writing up my thesis. Had a mental breakdown, life is a lie, etc.

This is almost my situation. Although I'm only doing a master's degree and there's a good chance that I'll be able to temporarily withraw from the program and re-enter if or when I'm "better" so that I can submit my thesis and graduate.

I sympathize with you, user. Everyone in your life feeds you lies from the moment you're born about how you just need to stay in school, try really hard and get good grades and then an amazing well-paying career will be waiting for you at the end.

No one tells you that 95% of your time in grad school will be spent grading undergrads' papers, fixing broken lab equipment and dealing with delays and administrative red tape.

The professors for the most part don't really care, as long as they keep getting fresh grad students signed on every year so that they can get more grant money and use them as slave labour to teach their classes for them and run their research labs.

Should have just become a building contractor straight out of high school. Really. What's the point.

As a doctor or a patient?

OP here. This. And don't fall for the "studying STEM will land you a well paid job" meme. It's (T)EM at best.

This

What does STEM stand for

Ok I laughed a little nice one but the doctor of course.

That guy on youtube drinking jars of gene replacement fluid he boight online in order to get ripped. . .he's an idiot, right?

Link?

My question is this: If you know most of it is bullshit anyway, why not just get on the bandwagon and market some kind of "miracle cure" to unsuspecting Americans. they don't know any better and, chances are, you can cash out before the Feds crack down on the nonsense (if they ever do). Make a quick buck and never look back. And if the Feds ever do come knocking, leave the country and never look back.

Because you need certain skills as a snake oil salesman (which also will make you an successful scientist, incidentally) and I do not possess these skills

Don't know. As a nursing student, I've seen many patients who get referred to psychiatrists and I think the number of mental illness diagnoses are on the rise.
I can see potential in your field. I'd say volunteer in a psychiatric unit and see for yourself.

No i dont have the link. I know he is an idiot, so nevermind that. So my wife and I have a few more embryos in cryostorage. Not planning to have more kids. Why is it I can't use these for stem cells so I can live forever?

You UK? Manchester cancer research UK labs are hiring a shit ton of you stem cell guys atm.
T. Antibody biotechnician

You should've gone down the path of immunology like me. I really enjoy my job. Syd/Australia

That's what a frontman is for. Get someone who can sell, give him a cut, and make a killing. Look up South Florida Pain Clinic. Granted it's not the same situation but the idea is generally the same. Doctor does all the "scientific/prescribing" bullshit, frontman hosts the clinics. Bonus points if you can get something that a type of addict will pay for. Everyone wants to live forever, just go from there. You just need to know when to get out before the Feds ass rape you with their "laws."

First of all, they would be rejected since they carry half your wifes DNA. Second, they can not expanded indefinitely, Hayflick limit and shit. Third even you would have an unending pool of autologous pluripotent stem cells, they will not simply replace all you damaged or raging tissue.

I'm open to all of Western and Northern Europe. So I will take a look.

I'm about to graduate with a biology major and a minor in writing. I'm aiming for a PhD, possibly in infectious diseases. I want to work at the CDC. Do you see the situation any better in that direction?

My passion is in writing, but I decided to go into STEM as my career instead because I always found it interesting, and I knew that trying to start up a writing career would probably kill my passion, once I knew I had to survive off of it. I'm hoping my experience in the sciences will provide a stable income and also inform my fiction writing. I don't want to be a writer that knows only writing, just like I don't want to be a biologist that only knows biology.

What do you think of Sir Tim Hunt? Do you think he really genetically cured cancer? Is that why he was run out of academia and then we get half assed genetic "treatments" instead of a cure?

you don't genetically "cure" cancer. Cancer is unregulated prolific growth caused by mutations. Cancer can thus be caused in many different ways, and manifest in many different forms. There will never be -a- cure for cancer. There can only be better treatments, and preventative measures taken against certain kinds of cancer based on genetic predisposition.

CRISPR* for those confused.

Unless you're premed. I just graduated with a degree in bio and can't for the life of me imagine what use it would have outside of entering medicine

Hmm..so how about this - They won't make me live forever, but I only have to make it until more ambitious/talented individuals than yourself figure out how pluripotent stem cells can let us make it to 500. So is it at least plausible that can stay healthy enough until such a time by keep supplying myself with embryonic stem cells as I go?
I need some hope, OP, I'm late 30's and only starting to build my empire. Was a late bloomer and I recon I need at least 200 years in order to rule the world.

> Doesn't realise it's precisely the politically correct cuck-class who are doing this.